Outlanders in an unusual habitat : Holothuria mammata (Grube, 1840) behaviour on seagrass meadows from Ria Formosa (S Portugal)

Holothuria mammata is one of the new target species from the Mediterranean and Atlantic. Usually, it inhabits rocky bottoms, staying in crevices and holes during the day and leaving them in the night for feeding on sandy bottoms. However, it can be found in unusual habitats such as seagrass with diurnal and nocturnal feeding. This study provides information for the first time on the behaviour, density and small scale distribution of H. mammata in a seagrass habitat from Ria Formosa (S Portugal). To reach these aims, a mark/recapture methodology was used. Abundance was estimated through R statistical software v.2.15.3 (package “Rcapture”). The minimum area method was applied in GRASS GIS v.6.4.2 for home range. Size distribution was estimated applying a ShapiroWilk test. Rayleigh test for randomness was applied to study the directionality of movements. A circular one-way ANOVA was used to test for differences in movement direction. Capture probability was higher on seagrass than sand and the total length of the individuals ranged from 13 to 25cm. Movement speed was between 4.7 and 14.7 m day-1. Movements were not directional. H. mammata differs in its behaviour from the related Holothuria arguinensis occurring in the same habitat

The color dependent morphology of the post-AGB star HD161796

Context. Many protoplanetary nebulae show strong asymmetries in their surrounding shell, pointing to asymmetries during the mass loss phase. Questions concerning the origin and the onset of deviations from spherical symmetry are important for our understanding of the evolution of these objects. Here we focus on the circumstellar shell of the post-AGB star HD 161796. Aims. We aim at detecting signatures of an aspherical outflow, as well as to derive the properties of it. Methods. We use the imaging polarimeter ExPo (the extreme polarimeter), a visitor instrument at the William Herschel Telescope, to accurately image the dust shell surrounding HD 161796 in various wavelength filters. Imaging polarimetry allows us to separate the faint, polarized, light from circumstellar material from the bright, unpolarized, light from the central star. Results. The shell around HD 161796 is highly aspherical. A clear signature of an equatorial density enhancement can be seen. This structure is optically thick at short wavelengths and changes its appearance to optically thin at longer wavelengths. In the classification of the two different appearances of planetary nebulae from HST images it changes from being classified as DUPLEX at short wavelengths to SOLE at longer wavelengths. This strengthens the interpretation that these two appearances are manifestations of the same physical structure. Furthermore, we find that the central star is hotter than often assumed and the relatively high observed reddening is due to circumstellar rather than interstellar extinction.Comment: Accepted for publication in A&

Osteochondral transfer using a transmalleolar approach for arthroscopic management of talus posteromedial lesions

SummaryCharacterizing osteochondral lesions of the talus has enabled the strategies of surgical management to be better specified. The main technical problem is one of access for arthroscopy instruments to posteromedial lesions. A range of techniques and approaches has been described in ankle arthroscopy in general, and a transmalleolar approach provides reliable and efficient access in these cases. It is frequently used for transchondral drilling, but also enables satisfactory implant positioning in autologous osteochondral mosaicplasty procedures. We report our technique and results on five cases with a minimum 1.2 years’ follow-up

The inner environment of Z~CMa: High-Contrast Imaging Polarimetry with NaCo

Context. Z\,CMa is a binary composed of an embedded Herbig Be and an FU Ori class star separated by $\sim100$ au. Observational evidence indicate a complex environment in which each star has a circumstellar disk and drives a jet, and the whole system is embedded in a large dusty envelope. Aims. We aim to probe the circumbinary environment of Z\,CMa in the inner 400 au in scattered light. Methods. We use high contrast imaging polarimetry with VLT/NaCo at $H$ and $K_s$ bands. Results. The central binary is resolved in both bands. The polarized images show three bright and complex structures: a common dust envelope, a sharp extended feature previously reported in direct light, and an intriguing bright clump located 0\farcs3 south of the binary, which appears spatially connected to the sharp extended feature. Conclusions.We detect orbital motion when compared to previous observations, and report a new outburst driven by the Herbig star. Our observations reveal the complex inner environment of Z\,CMa with unprecedented detail and contrast.Comment: Accepted for publication in A&A Letter

Metabolic channeling of phe for lignin biosynthesis in maritime pine

Phenylalanine (Phe) is the main precursor of phenylpropanoids biosynthesis in plants. This vast family of Phe-derived compounds can represent more than 30% of captured photosynthetic carbon, playing essential roles in plants such as cell wall components, defense molecules, pigments and flavors. In addition to its physiological importance, phenylpropanoids and particularly lignin, a component of wood, are targets in plant biotechnology. The arogenate pathway has been proposed as the main pathway for Phe biosynthesis in plants (Maeda et al., 2010). The final step in Phe biosynthesis, catalyzed by the enzyme arogenate dehydratase (ADT), has been considered as a key regulatory point in Phe biosynthesis, due to its key branch position in the pathway, the multiple isoenzymes identified in plants and the existence of a feedback inhibition mechanism by Phe. So far, the regulatory mechanisms underlying ADT genes expression have been poorly characterized, although a strong regulation of the Phe metabolic flux should be expected depending on its alternative use for protein biosynthesis versus phenylpropanoid biosynthesis. This second fate involves a massive carbon flux compared to the first one. Here we report our current research activities in the transcriptional regulation of ADT genes by MYB transcription factors in Pinus pinaster. The conifers channels massive amounts of photosynthetic carbon for phenylpropanoid biosynthesis during wood formation. We have identified the complete ADT gene family in maritime pine (El-Azaz et al., 2016) and a set of ADT isoforms specifically related with the lignification process. The potential control of transcription factors previously reported as key regulators in pine wood formation (Craven-Bartle et al., 2013) will be presented.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Constraining the mass of the planet(s) sculpting a disk cavity. The intriguing case of 2MASS J16042165-2130284

The large cavities observed in the dust and gas distributions of transition disks may be explained by planet-disk interactions. At ~145 pc, 2MASS J16042165-2130284 (J1604) is a 5-12 Myr old transitional disk with different gap sizes in the mm- and $\mu$m-sized dust distributions (outer edges at ~79 and at ~63 au, respectively). Its $^{12}$CO emission shows a ~30 au cavity. This radial structure suggests that giant planets are sculpting this disk. We aim to constrain the masses and locations of plausible giant planets around J1604. We observed J1604 with the Spectro-Polarimetric High-contrast Exoplanet REsearch (SPHERE) at the Very Large Telescope (VLT), in IRDIFS\_EXT, pupil-stabilized mode, obtaining YJH- band images with the integral field spectrograph (IFS) and K1K2-band images with the Infra-Red Dual-beam Imager and Spectrograph (IRDIS). The dataset was processed exploiting the angular differential imaging (ADI) technique with high-contrast algorithms. Our observations reach a contrast of $\Delta K, YH$ ~12 mag from 0.15" to 0.80" (~22 to 115 au), but no planet candidate is detected. The disk is directly imaged in scattered light at all bands from Y to K, and it shows a red color. This indicates that the dust particles in the disk surface are mainly $\gtrsim0.3\,\mu$m-sized grains. We confirm the sharp dip/decrement in scattered light in agreement with polarized light observations. Comparing our images with a radiative transfer model we argue that the southern side of the disk is most likely the nearest. This work represents the deepest search yet for companions around J1604. We reach a mass sensitivity of $\gtrsim 2-3M_{Jup}$ from ~22 to ~115 au according to a hot start scenario. We propose that a brown dwarf orbiting inside of ~15 au and additional Jovian planets at larger radii could account for the observed properties of J1604 while explaining our lack of detection.Comment: 10 pages, 7 Figures. Accepted for publication in A&A . Abridged abstrac

Nitrogen metabolism in forest trees

NITROGEN METABOLISM IN FOREST TREES Francisco Cánovas, Concepción Ávila, Fernando N de la Torre, Rafael Cañas, Belén Pascual. Universidad de Málaga Email:[email protected] Forests are essential components of the ecosystems covering approximately one-third of the Earth’s land area and playing a fundamental role in the regulation of terrestrial carbon sinks. Forest trees are also of significant economic importance, as they are used for timber and paper production worldwide. A sustainable management of forest resources is needed to preserve natural forest and to meet the increasing international demands in the production of wood and the other forest-derived products. New advances and developments in biotechnology will contribute to accelerate the domestication of important traits for forest productivity. It is critical to identify the fundamental constraints on forest productivity to addressing these constraints with modern genomic tools. Nitrogen availability extremely low in forest ecosystems, and consequently, forest trees have evolved adaptive mechanism and biotic interactions to guarantee the strict economy of this essential nutrient. Nitrogen assimilation and recycling play a key role in the tree growth and biomass production and we firmly believe that knowledge on nitrogen metabolism will lead to approaches aimed at increasing forest productivity. In our laboratory, we are interested in studying nitrogen metabolism and its regulation the conifer maritime pine (Pinus pinaster Aiton), a forest tree species of great economic and ecological importance in the Mediterranean area and relevant model for conifer genomic research in Europe. Current research efforts are focused on improving the understanding of the response of conifer trees to ammonium availability and the transcriptional control of ammonium assimilation into amino acids. An overview and update of our research programme will be presented and discussed. Research supported by Spanish Ministry of Economy and Competitiveness and Junta de Andalucía (Grants BIO2012-33797, PLE2009-016 and research group BIO-114).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Direct imaging of a massive dust cloud around R Coronae Borealis

We present recent polarimetric images of the highly variable star R CrB using ExPo and archival WFPC2 images from the HST. We observed R CrB during its current dramatic minimum where it decreased more than 9 mag due to the formation of an obscuring dust cloud. Since the dust cloud is only in the line-of-sight, it mimics a coronograph allowing the imaging of the star's circumstellar environment. Our polarimetric observations surprisingly show another scattering dust cloud at approximately 1.3" or 2000 AU from the star. We find that to obtain a decrease in the stellar light of 9 mag and with 30% of the light being reemitted at infrared wavelengths (from R CrB's SED) the grains in R CrB's circumstellar environment must have a very low albedo of approximately 0.07%. We show that the properties of the dust clouds formed around R CrB are best fitted using a combination of two distinct populations of grains size. The first are the extremely small 5 nm grains, formed in the low density continuous wind, and the second population of large grains (~0.14 {\mu}m) which are found in the ejected dust clouds. The observed scattering cloud, not only contains such large grains, but is exceptionally massive compared to the average cloud.Comment: 8 pages, 7 figures published in A&

Non-Coding RNA Sequencing of Equine Endometrium During Maternal Recognition of Pregnancy.

Maternal recognition of pregnancy (MRP) in the mare is not well defined. In a non-pregnant mare, prostaglandin F2α (PGF) is released on day 14 post-ovulation (PO) to cause luteal regression, resulting in loss of progesterone production. Equine MRP occurs prior to day 14 to halt PGF production. Studies have failed to identify a gene candidate for MRP, so attention has turned to small, non-coding RNAs. The objective of this study was to evaluate small RNA (<200 nucleotides) content in endometrium during MRP. Mares were used in a cross-over design with each having a pregnant and non-mated cycle. Each mare was randomly assigned to collection day 11 or 13 PO (n = 3/day) and endometrial biopsies were obtained. Total RNA was isolated and sequencing libraries were prepared using a small RNA library preparation kit and sequenced on a HiSeq 2000. EquCab3 was used as the reference genome and DESeq2 was used for statistical analysis. On day 11, 419 ncRNAs, representing miRNA, snRNA, snoRNA, scaRNA, and vaultRNA, were different between pregnancy statuses, but none on day 13. Equine endometrial ncRNAs with unknown structure and function were also identified. This study is the first to describe ncRNA transcriptome in equine endometrium. Identifying targets of these ncRNAs could lead to determining MRP